1. Persons undertaking dealings in the facility with GMOs requiring PC2 containment must comply with these Behavioural Requirements.
Non-GMOs, exempt dealings and PC1 dealings in the facility
2. Persons undertaking work in the facility on non-GMOs, exempt dealings or dealings which may be undertaken in a PC1 facility must comply with these Behavioural Requirements unless:
- procedures are implemented to ensure that non-GMOs, exempt dealings or dealings which may be undertaken in a PC1 facility, are not cross-contaminated with GMO dealings requiring containment in a PC2 facility;
- the above procedures are documented; and
- the primary and any secondary container used to transport any organism out of the facility must be free of contamination with GMOs prior to being transported out of the facility.
- NOTE: Means of preventing cross-contamination could include physical separation of the work, or separation by working at different times and ensuring any contaminated surfaces are decontaminated prior to working with a different organism.
Doors & windows
3. Except during the entry and exit of personnel, supplies and/or equipment, doors of the facility must be closed while procedures with GMOs are being conducted. Entrance doors into the facility must remain locked, or the facility must be otherwise secured, when facility personnel are not in attendance.
4. Dedicated “Emergency Only” exits must not be used to enter nor exit the facility except in an emergency.
5. Windows must remain closed and locked, or otherwise secured, while procedures with GMOs are being conducted or when facility personnel are not in attendance.
6. Where any Class I or Class II biological safety cabinet is installed and used for procedures with GM micro-organisms, it must be used and decontaminated in accordance with the requirements of AS 2252.4.
7. Where any Class I or Class II biological safety cabinet is installed and used for procedures with GMOs, it must be used and decontaminated in accordance with the requirements of AS 2252.4.
Personal protective equipment
8. If the work in the facility involves GM micro-organisms or GMOs that could persist on clothing or persons the following personal protective equipment must be worn by personnel undertaking dealings in the facility:
- protective clothing to afford protection to the arms and the body; and
- disposable gloves, when dealing with GM micro-organisms, including viral vectors where the parent organism satisfies the criteria for classification as a Risk Group 2 organism under AS/NZS 2243.3.
- NOTE: Consideration should be given to the wearing of appropriate forms of eye protection.
10. Personal protective equipment, with the exception of gloves, may be worn if moving directly to another containment facility, certified to at least PC2 by the Regulator, that is directly connected to the facility or is connected by a corridor, stairs or other space that is not a public thoroughfare and in which there is negligible risk of the release of the GMOs or of cross-contamination should other personnel be encountered or contacted in the corridor.
11. Decontamination must be undertaken in accordance with Section 3.1 of the Regulator’s Guidelines for the Transport, Storage and Disposal of GMOs as in force from time to time unless otherwise approved in writing by the Regulator.
12. All decontamination procedures conducted inside the facility must be carried out by trained personnel.
13. GMOs, non-GMOs containing GMOs or any waste containing GMOs must be decontaminated prior to disposal if the method of disposal is not also the method of decontamination.
- NOTE: Where appropriate, visual inspection of the container(s) may be used to confirm whether decontamination is necessary (e.g. in the case of macroscopic GMOs which are visible to the naked eye).
- NOTE: Decontamination of liquid effluent may be by filtration, if it is effective in removing the GMOs from the effluent.
16. Equipment contaminated with or suspected to be contaminated with GMOs must be decontaminated before being removed from the facility, except if the equipment is being transported for the purposes of decontamination in accordance with the Regulator’s Guidelines for the Transport, Storage and Disposal of GMOs, as in force from time to time, and other relevant guidelines issued by the Regulator.
17. Personal protective equipment contaminated with or suspected to be contaminated with GMOs must be taken off as soon as practicable and decontaminated prior to reuse or disposal. Protective clothing that is known to be not contaminated with GMOs may be washed using normal laundry methods. Gloves must be disposed of after use and prior to exiting the facility.
18. Persons who have been performing procedures in the facility that involve GM micro-organisms, or who have had hand contact with GMOs that could persist on the hands after exit from the facility, must decontaminate their hands before leaving the facility.
- NOTE: This may include the use of soap and water, if appropriate. If wash-basins are to be used, the use of hand operated taps is not acceptable, as they are a ready source of contamination. Soap and other decontamination agents should be dispensed from hands free dispensers.
Escape/Spills of GMOs
19. If a GM aquatic organism or aquatic organism containing GMOs escapes within the facility, the aquatic organism must be captured and returned to its container, or euthanased.
20. Documented procedures must be in place to decontaminate any spills involving GMOs inside the facility. The procedures must be made available to the Regulator if requested.
21. If a spill of GMOs or any material containing GMOs occurs inside the facility, the spills procedures must be implemented to decontaminate the spill as soon as reasonably practicable.
22. In the event of the escape, unintentional release, spill, leak, or loss of GMOs outside of the facility:
- efforts must be implemented as soon as reasonably practicable to locate and/or retrieve the GMOs and return the GMOs to containment or render them non-viable; and
- the incident must be reported to the Regulator as soon as practicable.
24. All containers of GMOs must be clearly labelled so as to indicate that they contain GMOs. Any unlabelled material must be treated as a GMO and handled in accordance with these requirements.
- NOTE: Labelling enables the separation of GM work from non-GM work and enhances the control of GMOs within the facility.
Removal and storage of GMOs
25. Transport and storage of all GMOs outside of the facility must be conducted in accordance with the Regulator’s Guidelines for Transport, Storage and Disposal of GMOs, as in force from time to time, and other relevant guidelines issued by the Regulator.
26. All cultures of GMOs being stored inside the facility must be sealed during storage to prevent dissemination of the GMOs.
- NOTE: The type of container necessary to prevent the GMOs from escaping will vary depending on the type of organisms being stored.